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Part-Time Faculty Pool – Biotechnology & Biomanufacturing
Location: Shoreline Community College, WA
Job Number: 00799-1 (posted 03/27/2024)
Course Name: BIOL 288 Basic Principles of Flow Cytometry
Description: This course will present the theory of flow cytometry as well as provide practical laboratory experience using the instrumentation. Students will receive hands-on training calibrating and troubleshooting a flow cytometer, preparing and staining cells for analysis, data collection and analysis of data.
Course Outcomes:
- Understand and apply the theory of flow cytometry, use of the instrumentation and associated software.
- Calibrate and troubleshoot the flow cytometer using fluorescent bead standards.
- Design, perform and analyze data from cell staining experiment using flow cytometry.
Topic Outline:
- Applications and principles of flow cytometry including fluidics, electronics, optics, and software applications
- Routine maintenance, calibration, and data acquisition
- Staining protocols:
- Cell fixation, blocking, and staining optimization using fluorescent probes
- Staining cell surface markers
- Nuclear staining
- Cell cycle analysis
- Two color staining
- Analysis of stained cells
- Design appropriate controls & set up cytometer to detect stained cells, compensate where needed if using two color staining
- Collect and analyze data